Abstract The increased worldwide demand for chicken meat requires constant automation of operating systems and the proper implementation of self-control programs by slaughterhouses. Thus, this study aimed to evaluate the efficacy of a self-washing system using heated water on reducing the load of public health concern bacteria on a broiler feet-cutter disc. In a three-month period, 84 samples (n = 42 per group) were collected for microbiological analysis from the feet-cutter disc with an operational self-washing process with Non-Heated Water (NHW) and Heated Water (HW). The logarithmic counts of Mesophilic Aerobics, Sulphite-Reducing Clostridia (SRC), Escherichia coli and Staphylococcus aureus in the NHW group were 1.82, 0.56, 0.31 and 0.14 Log10 CFU/cm2, respectively, whereas in the HW group were 1.17, 0.04, 0.15 and 0.08 Log10 CFU/cm2. Salmonella spp. was absent in all evaluated samples in both groups. The heated-water system reduced bacterial count, but statistical differences were solely observed for Mesophilic Aerobics and SRC. Nevertheless, its usage could be an additive in the operational procedure to avoid microbiological violations of food safety, in addition to being easy-to-implement and affordable for the industry.

Resumo O aumento mundial da demanda por carne de frango exige constante automação de sistemas de operação e implementação adequada de programas de autocontrole pelos abatedouros. Assim, o objetivo do presente estudo foi avaliar a eficiência de sistema de autolavagem com água aquecida na redução da contagem de bactérias de interesse em saúde pública em disco corta-pés de frangos. Em um período de três meses, 84 amostras (n = 42, por grupo) foram colhidas para análises microbiológicas em disco corta-pés contando com um sistema operacional de autolavagem com água não aquecida (NHW) e água aquecida (HW). As contagens de Aeróbios Mesófilos, Clostridium Sulfito-redutor, Escherichia coli e Staphylococcus aureus, no grupo NHW, foram de 1,82, 0,56, 0,31 e 0,14 Log10 UFC/cm2, respectivamente, enquanto que, no grupo HW, foram de 1,17, 0,04, 0,15 e 0,08 Log10 UFC/cm2. Salmonella spp. foi ausente em todas amostras avaliadas, em ambos os grupos. O sistema com água aquecida reduziu as contagens bacterianas, mas diferenças estatísticas foram observadas somente em Aeróbios Mesófilos e Clostridium Sulfito-redutor. Apesar disso, seu uso pode ser um adicional ao procedimento operacional para evitar a violação dos limites microbiológicos de segurança alimentar, além de ser um sistema de fácil implementação e de baixo custo.

Abstract The neonatal period represents a critical stage for the establishment and development of the gut microbiota, which profoundly influences the future health trajectory of individuals. This review examines the importance of intestinal microbiota in humans and dogs, aiming to elucidate the distinct characteristics and variations in the composition between these two species. In humans, the intestinal microbiota contributes to several crucial physiological processes, including digestion, nutrient absorption, immune system development, and modulation of host metabolism. Dysbiosis, an imbalance or disruption of the gut microbial community, has been linked to various disorders, such as inflammatory bowel disease, obesity, and even neurological conditions. Furthermore, recent research has unveiled the profound influence of the gut-brain axis, emphasizing the bidirectional communication between the gut microbiota and the central nervous system, impacting cognitive function and mental health. Similarly, alterations in the canine intestinal microbiota have been associated with gastrointestinal disorders, including chronic enteropathy, such as inflammatory bowel disease, food allergies, and ulcerative histiocytic colitis. However, our understanding of the intricacies and functional significance of the intestinal microbiota in dogs remains limited. Understanding the complex dynamics of the intestinal microbiota in both humans and dogs is crucial for devising effective strategies to promote health and manage disease. Moreover, exploring the similarities and differences in the gut microbial composition between these two species can facilitate translational research, potentially leading to innovative therapeutic interventions and strategies to enhance the well-being of both humans and dogs. individuals processes digestion absorption metabolism Dysbiosis community disorders disease obesity conditions Furthermore gutbrain brain axis Similarly enteropathy allergies colitis However limited Moreover wellbeing well being

Abstract The aim of this study was to investigate the presence of virulence and antimicrobial resistance genes and clonal profile of Enterobacteriaceae isolated from calves, cows, feeding buckets and the milk bucket. A a total of 31 Enterobacteriaceae isolates from calves (6), milk bucket (6), feeding buckets (6) and from cows’ rectum (13) were used. The presence of LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge as well as the antimicrobial resistance genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC and MCR-1 and SH were evaluated by PCR. The LT toxin gene were detected in five isolates (16.1%) and the mrkD gene was detected in three isolates (9.0%). The CTX-M-1 gene was detected in 13 isolates (41.9%), CTX-M-2 in five isolates (16.1%) and ACC-M in four isolates (12.9%). Most of the isolates obtained demonstrated, resistance to cephalothin (87.5%), ampicillin, (87.5%) and streptomycin (84.3%) with multidrug resistance observed in all isolates. Isolates from bucket and cow’s rectum, calves and milk bucket, calf and cow’s rectum shared the same pulsotypes. These findings may suggest that enterobacteria carring virulence and resistance genes may persist in the environment and became a reservoir of these genes.

Abstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer's health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness.

ABSTRACT The aim of this study was to evaluate the feasibility of using ethanol (AE) and an aqueous sodium chloride solution 30% (ASCS) in various time periods for the preparation of carcasses of cats for the teaching of surgical techniques. The corpses of 60 cats were used. The specimens were fixed in AE, according to group, for 30 days (G1), 30 and 60 days (G2) and 30, 60 and 90 days (G3), and ASCS was applied to all of them at 30, 60, 90 and 120 days of conservation and the result was measured by means of the biomechanical study in the tissues to determine the best moment to interrupt the fixation in AE using as control the greatest similarity in tissue resistance found in fresh cadavers. The use of the anatomicaltechniqueusingAE andASCSwas efficientthroughout theexperiment.There was no significant difference between the mean value of the maximum breaking force (N) or between the storage groups in the ASCS and the control group in any of the groups for the skin samples. Neither was there a significant difference in maximum force between fixation and preservation groups, compared to the control group in the groups for jejunum samples. However, G2 presented less variation in the breaking force (-0.21 mm), being, therefore, the group most similar to fresh animals. The anatomical technique used was found to be efficient for both fixation and preservation of cat carcasses for up to seven months.

RESUMEN El objetivo del estudio fue evaluar la factibilidad de utilizar alcohol etílico (AE) y una solución acuosa de cloruro de sodio al 30% (SACS) en varios periodos de tiempo para la preparación de cadáveres de gatos para la enseñanza de técnicas quirúrgicas. Se utilizaron los cadáveres de 60 gatos. Los especímenes fueron fijados en AE, según grupo, por 30 días (G1), 30 y 60 días (G2) y 30, 60 y 90 días (G3), y a todos se les aplicó SACS a los 30, 60, 90 y120 días de conservación y el resultado fue medido mediante el estudio biomecánico en los tejidos para determinar el mejor momento para interrumpir la fijación en AE, utilizando como control la mayor similitud en la resistencia tisular encontrada en cadáveres frescos. El uso de la técnica anatómica utilizando AE y SACS fue eficiente durante todo el experimento. No hubo diferencia significativa entre las medias de la fuerza máxima de ruptura (N) ni entre los grupos de almacenamiento en el SACS y el tiempo de control en ninguno de los grupos para las muestras de piel. Tampoco se observó diferencia significativa en la fuerza máxima entre los grupos de fijación y preservación, comparado al grupo control en los grupos para las muestras de yeyuno. No obstante, G2 presentó menor variación en la fuerza de ruptura (-0.21 mm), siendo, por lo tanto, el grupo más parecido a los animales frescos. Se encontró que la técnica anatómica utilizada es eficiente tanto para la fijación y para la conservación de cadáveres de gatos por hasta siete meses.

RESUMO: A indústria avícola brasileira gera grandes quantidades de resíduos orgânicos, como a cama de frango, utilizada frequentemente na agricultura. Entre as bactérias presentes neste fertilizante orgânico estão os membros da família Enterobacteriaceae, entre eles a Escherichia coli. O objetivo deste estudo foi analisar o perfil de fatores de virulência e resistência antimicrobiana de isolados de E. coli provenientes do fertilizante orgânico aviário. Um total de 47 cepas de E. coli foram testadas por meio da reação em cadeia da polimerase para detectar genes de virulência (hlyF, iss, ompT, iutA e iroN). Quatorze antimicrobianos foram utilizados para testar a susceptibilidade antimicrobiana nos isolados. Genes característicos de E. coli Patogênica Aviária (APEC) foram encontrados entre os isolados, sendo os genes hlyF, iss e ompT os mais prevalentes. Os isolados apresentaram alta resistência (˃50%) à tetraciclina, gentamicina, cefotaxima, nitrofurantoína, trimetoprima-sulfametoxazole e ampicilina. Múltipla resistência a drogas antimicrobianas foi encontrada em grande número de isolados (>70%). Os resultados obtidos mostraram a presença de células APEC portando genes de virulência e resistência a antimicrobianos após 15 dias de processo de empilhamento nas granjas, indicando que o processo necessita de um aperfeiçoamento para eliminar estas células.

ABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the most prevalent. The isolates showed high resistance (˃50%) to tetracycline, gentamicin, cefotaxime, nitrofurantoin, trimethoprim-sulfamethoxazole and ampicillin. Multidrug resistance was reported in a great number of strains (>70%). The results showed the presence of APEC cells with virulence genes and antimicrobial resistance after 15 days of the windrowing process in poultry houses, it means this process should be improved to eliminate these cells.

ABSTRACT Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.

Abstract Avian pathogenic Escherichia coli (APEC) isolates from apparently healthy free range helmeted guineafowl were characterized. Most of them had a high frequency of virulence associated genes, multi drug resistance and high pathogenicity. We demonstrated that helmeted guineafowl have potential to transmit antibiotic resistant APEC to other species including humans.

ABSTRACT In this experiment, the beneficial effects of mannan-oligosaccharide (MOS) on immunology and intestinal microbiology of Nile tilapia juveniles was demonstrated. Prior to this, three levels of MOS in Nile tilapia diets (1, 8, and 15 g.kg−1) were tested, and hematological parameters, serum lysozyme, and intestinal microbiology were analyzed. The fish blood was sampled at day zero (basal sample) and after 45 days of trial, and the intestinal microbiota was evaluated at the end of the experiment. After 45 days of trial, fish fed 8 and 15 g.kg−1 of MOS presented an increase in both aerobic and lactic acid bacteria numbers in their guts. The MOS feeding also increased the counts of total leukocytes, monocytes, and lymphocytes of fish, but a decrease in neutrophils was also observed. Additionally, the serum lysozyme was higher in all fish fed MOS. The dietary MOS is able to modulate the intestinal microbiota, increasing the number of beneficial bacteria, and immunostimulates the Nile tilapia juvenile, giving rise to white blood cells and serum lysozyme.

O consumo de carne ovina tem aumentado nos últimos anos, no Brasil. Entretanto, pouca informação sobre a qualidade desse produto está disponível. Com o intuito de avaliar as condições higiênico-sanitárias das carcaças ovinas, foram realizadas a quantificação das populações de microrganismos indicadores (mesófilos e psicrotróficos; coliformes totais e termotolerantes; Escherichia coli; bolores e leveduras) e a identificação de microrganismos patogênicos (Salmonella sp. and Listeria spp.). Um total de 60 carcaças foram amostradas em um frigorífico, em São Paulo, entre fevereiro e dezembro de 2006. Suabes foram coletados em três pontos (dianteiro, lombo e traseiro), na superfície muscular das carcaças, após a lavagem final destas. A análise estatística consistiu na avaliação descritiva dos resultados cujas contagens foram agrupadas em intervalos populacionais. As contagens variaram de 1,0 x 10¹ a 8,0 x 10(4)UFC cm-2 para mesófilos; de 1,0 x 10(0) a 4,4 x 10(4)UFC cm-2 para psicrotróficos; de < 1,0 x 10(0) a 4,4 x 10(4)UFC cm-2 para bolores e leveduras; de < 0,3 a > 32,0 NMP cm-2 para coliformes totais e termotolerantes; e Escherichia coli. Salmonella sp. e Listeria spp. não foram detectadas em nenhuma das amostras coletadas nas carcaças. A maioria das carcaças apresentou baixas contagens para todas as categorias de microrganismos. Os resultados encontrados podem ser explicados pelo pequeno tamanho da indústria onde o trabalho foi realizado e sugerem que carne ovina de boa qualidade microbiológica pode ser obtida. No entanto, melhorias nas condições higiênico-sanitárias ainda são necessárias.

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.

Com o objetivo de analisar as características físico-químicas do leite UAT durante sua industrialização e de comparar os resultados com a legislação vigente, foram analisadas 150 amostras, colhidas em seis etapas, em diferentes fases do processamento, sendo 60 de leite cru, 60 de leite pasteurizado e 30 de leite UAT. As amostras foram submetidas à determinação de acidez titulável, densidade, teor de gordura, extrato seco total (EST) e desengordurado (ESD), ponto crioscópico, pH, redutase, estabilidade ao álcool 68% e à pesquisa das enzimas peroxidase e fosfatase alcalina. Os resultados indicaram que os valores médios do extrato seco total e do extrato desengordurado, bem como do índice crioscópico apresentaram-se, em algumas colheitas, inferiores aos estabelecidos pela legislação vigente, indicando possível aumento no teor de água do leite.

With the aim of analyzing the physicochemical characteristics of UHT milk during its industrialization and comparing the results with the Brazilian legislation, 150 samples collected in six stages, in different processing phases, were analyzed, being 60 samples of raw milk, 60 of pasteurized milk and 30 of UHT milk. The samples were submitted to the analysis of Dornic acidity, density, fat content, total solids, solids-non-fat, freezing point, pH, methylene blue reduction, stability to 68% alcohol and screening for peroxidase and alkaline phosphatase enzymes. The results indicated that the mean values of total solids and solids-non-fat and freezing point, were in some collections, lower than the limits established in the Brazilian legislation, demonstrating a possible increase in milk water content.